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Syndecan binding protein Rabbit Monoclonal Antibody [KD Validated]
Datasheet   

  • Catalog Number:

    E46F61201

  • Amount:

    40ul

  • Cite ID#:

    EN5547639

  • Price:

    295$

  • Gene Name:

    SDCBP

  • Purification:

    The antibody was purified by immunogen affinity chromatography.

  • Storage Buffer:

    Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

  • Storage/Stability:

    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

  • Swiss-Prot No.:

    O00560

  • Form:

    Liquid

  • Altername:

    SDCBP; Syndecan Binding Protein; MDA-9; SYCL; Syntenin-1; Pro-TGF-Alpha Cytoplasmic Domain-Interacting Protein 18; Syndecan-Binding Protein 1; Scaffold Protein Pbp1; TACIP18; SDCBP1; MDA9; Melanoma Differentiation Associated Protein-9; Melanoma Differentiation-Associated Protein 9; Syndecan Binding Protein (Syntenin); Syntenin; ST1

  • Immunogen:

    A synthesized peptide derived from human Syntenin

  • Reactivity:

    Human

  • Applications:

    Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)

  • Background:

    Gene Name: SDCBP, UniProt Entry: O00560, NCBI Gene Entry: 6386

  • Source:

    Rabbit

  • Antibody type:

    Rabbit monoclonal antibody

  • Isotype:

    Rabbit IgG

  • Molecular Weight:

    Predicted, 32 kDa, observed, 32 kDa

  • Recommended Dilutions:

    Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:2,000, Immunocytochemistry (IC): 1:1,000

  • Human Gene Id:

    6386

  • Western blotting analysis using anti-Syndecan binding protein antibody (E46F61201). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Syndecan binding protein antibody (E46F61201, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using ECL Substrate Kit .

  • Western blotting analysis using anti-Syndecan binding protein antibody (E46F61201). Syndecan binding protein expression in wild type (WT) and syndecan binding protein shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Syndecan binding protein antibody (E46F61201, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using ECL Substrate Kit .

  • Flow cytometric analysis of Syndecan binding protein expression in HepG2 cells using Syndecan binding protein antibody (E46F61201, 1:2,000). Green, isotype control; red, Syndecan binding protein.

  • Immunocytochemical staining of HepG2 cells with Syndecan binding protein antibody (E46F61201, 1:1,000). Nuclei were stained blue with DAPI; Syndecan binding protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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  • Amount:40ul
  • Price:295.00$

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