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EGF Protein, Mouse, Recombinant
Datasheet   

  • Catalog Number:

    E45M53424Y0-100

  • Amount:

    100 ug

  • Cite ID#:

    EN5423514

  • Price:

    156$

  • Type:

    Recombinant Proteins

  • Protein length:

    A DNA sequence encoding the mouse EGF (P01132) (Asn977-Arg1029) was expressed and purified.

  • Expression Host:

    Yeast

  • Species:

    Mouse

  • Amino acid sequence:

    Asn977-Arg1029

  • Tag:

    No tag

  • Molecular Weight:

    The recombinant mouse EGF consists of 53 amino acids and predicts a molecular mass of 6 KDa.

  • Activity:

    Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The EDfor this effect is typically 30-180 pg/mL.

  • Endotoxin:

    Please contact us for more information.

  • Gene Name:

    epidermal growth factor

  • Gene symbol:

    EGF

  • Swiss-Prot No.:

    P01132

  • Function:

    Diagnostic proteins, Growth Factor proteins, Biomarker Proteins, Enzyme Proteins, Biological Target proteins, Cell Therapy proteins, Cytokine Proteins

  • Summary:

    EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.

  • Form:

    Lyophilized

  • Purity:

    > 95 % as determined by SDS-PAGE.

  • Storage/Stability:

    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
    Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

  • Storage Buffer:

    Lyophilized from sterile PBS, pH 7.4.
    Please contact us for any concerns or special requirements.
    Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
    Please refer to the specific buffer information in the hard copy of CoA.

  • Synonyms:

    AI790464 Protein, Mouse

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  • Amount:100 ug
  • Price:156.00$

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