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DPP4/CD26 Protein, Cynomolgus, Recombinant (ECD, His Tag)
Datasheet   

  • Catalog Number:

    E45K53978M2-50

  • Amount:

    50 ug

  • Cite ID#:

    EN5424068

  • Price:

    1223$

  • Type:

    Recombinant Proteins

  • Research Field:

    Serine Proteases and Regulators

  • Protein length:

    A DNA sequence encoding the cynomolgus DPP4 (XP_005573375.1) (Asp34-Pro766) was expressed with a polyhistidine tag at the C-terminus.

  • Expression Host:

    HEK293 Cells

  • Species:

    Cynomolgus

  • Amino acid sequence:

    Asp34-Pro766

  • Tag:

    C-His

  • Molecular Weight:

    The recombinant cynomolgus DPP4 consists 744 amino acids and predicts a molecular mass of 86 kDa.

  • Activity:

    At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA.
    When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate.
    We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

  • Endotoxin:

    < 1.0 EU per μg protein as determined by the LAL method.

  • Gene Name:

    dipeptidylpeptidase 4

  • Gene symbol:

    DPP4

  • Protein Refseq:

    XP_005573375.1

  • Function:

    CD Molecule proteins, Enzyme Proteins

  • Summary:

    Dipeptidyl peptidase-4 (DPP4) or adenosine deaminase complexing protein 2 (ADCP 2) or T-cell activation antigen CD26 is a serine exopeptidase belonging to the S9B protein family that cleaves X-proline dipeptides from the N-terminus of polypeptides, such as chemokines, neuropeptides, and peptide hormones. The enzyme is a type II transmembrane glycoprotein, expressed on the surface of many cell types. It is also present in serum and other body fluids in a truncated form (sCD26/DPPIV). The soluble CD26 (sCD26) as a tumour marker for the detection of colorectal cancer (CRC) and advanced adenomas. As both a regulatory enzyme and a signalling factor, DPP4 has been evaluated and described in many studies. DPP4 inhibition results in increased blood concentration of the incretin hormones glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP). This causes an increase in glucose-dependent stimulation, resulting in a lowering of blood glucose levels. Recent studies have shown that DPP4 inhibitors can induce a significant reduction in glycosylated haemoglobin (HbA(1c)) levels, either as monotherapy or as a combination with other antidiabetic agents. Research has also demonstrated that DPP4 inhibitors portray a very low risk of hypoglycaemia development, and are a new pharmacological class of drugs for treating Type 2 diabetes.

  • Form:

    Lyophilized

  • Purity:

    > 95 % as determined by SDS-PAGE.

  • Storage/Stability:

    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
    Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

  • Storage Buffer:

    Lyophilized from sterile PBS, pH 7.4.
    Please contact us for any concerns or special requirements.
    Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
    Please refer to the specific buffer information in the hard copy of CoA.

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  • Amount:50 ug
  • Price:1223.00$

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