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Human GDF5/Growth/differentiation factor 5 ELISA Kit
Datasheet   

  • Catalog Number:

    E45K00643

  • Amount:

    96T

  • Cite ID#:

    EN5521094

  • Price:

    1009$

  • Type:

    Human ELISA Kits

  • Description:

    Human GDF5/Growth/differentiation factor 5 ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of GDF5 in biological samples. Our rigorously validated, ready-to-go Human GDF5/Growth/differentiation factor 5 ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect GDF5 in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.

    Precision
    Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
    Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.

  • Assay type:

    Sandwich

  • Detection principle:

    Spectrophotometrical

  • Reactivity:

    Human

  • Sample type:

    This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

  • Sensitivity:

    0.078 ng/mL

  • Assay range:

    0.156-10 ng/mL

  • Kit components:

    Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.

  • Other reagents required:

    Deionized or distilled water

  • Gene Name:

    GDF5

  • Swiss-Prot No.:

    P43026

  • Function:

    Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (PubMed:24098149, PubMed:21976273, PubMed:15530414, PubMed:25092592). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (PubMed:21976273). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (By similarity). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205).

  • Form:

    ELISA Kits

  • Storage/Stability:

    Store at +4°C. Please refer to protocols.

  • Synonyms:

    GDF-5,Bone morphogenetic protein 14,BMP-14,Cartilage-derived morphogenetic protein 1,CDMP-1,Lipopolysaccharide-associated protein 4,LAP-4,Radotermin,BMP14,CDMP1

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  • Amount:96T
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