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EphB4 Protein, Human, Recombinant (His Tag)
Datasheet   

  • Catalog Number:

    E45H51053M2-100

  • Amount:

    100 ug

  • Cite ID#:

    EN5421143

  • Price:

    624$

  • Type:

    Recombinant Proteins

  • Protein length:

    A DNA sequence encoding the extracellular domain (Met 1-Ala 539) of human EphB4 (NP_004435.3) precursor was expressed with a C-terminal polyhistidine tag.

  • Expression Host:

    HEK293 Cells

  • Species:

    Human

  • Amino acid sequence:

    Met1-Ala539

  • Tag:

    C-His

  • Molecular Weight:

    The recombinant human EphB4 is a monomeric protein. It consists of 535 amino acids and has a calculated molecular mass of 58.5 kDa. As a result of glycosylation, the rhEphB4 migrates as an approximately 72 kDa protein in SDS-PAGE under reducing conditions.

  • Activity:

    Measured by its binding ability in a functional ELISA. Immobilized human EphB4 at 2 μg/ml (100 μl/well) can bind human EphrinB2 with a linear range of 1-25 ng/ml.

  • Endotoxin:

    < 1.0 EU per μg of the protein as determined by the LAL method.

  • Gene Name:

    EPH receptor B4

  • Gene symbol:

    EphB4

  • Protein Refseq:

    NP_004435.3

  • Function:

    Kinase proteins, Growth Factor proteins, Receptor Proteins, Cytokine Proteins, Enzyme Proteins

  • Summary:

    Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

  • Form:

    Lyophilized

  • Purity:

    > 95 % as determined by SDS-PAGE.

  • Storage/Stability:

    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
    Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

  • Storage Buffer:

    Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.4
    Please contact us for any concerns or special requirements.
    Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
    Please refer to the specific buffer information in the hard copy of CoA.

  • Synonyms:

    HTK Protein, Human; MYK1 Protein, Human; TYRO11 Protein, Human

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  • Amount:100 ug
  • Price:624.00$

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