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VIP Protein, Human, Recombinant (His Tag)
Datasheet   

  • Catalog Number:

    E45H50684M2-100

  • Amount:

    100 ug

  • Cite ID#:

    EN5420774

  • Price:

    1223$

  • Type:

    Recombinant Proteins

  • Research Field:

    Neurotransmitter Associated Enzymes

  • Protein length:

    A DNA sequence encoding the human VIP (P01282-2) (Met1-Lys169) with a C-terminal polyhistidine tag was expressed.

  • Expression Host:

    HEK293 Cells

  • Species:

    Human

  • Amino acid sequence:

    Met1-Lys169

  • Tag:

    C-His

  • Molecular Weight:

    The recombinant human VIP comprises 160 amino acids and has a predicted molecular mass of 18.3 kDa. The apparent molecular mass of the protein is approximately 25, 21 and 15 kDa in SDS-PAGE under reducing conditions

  • Activity:

    At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA.
    When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate.
    We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

  • Endotoxin:

    < 1.0 EU per μg of the protein as determined by the LAL method.

  • Gene Name:

    vasoactive intestinal peptide

  • Gene symbol:

    VIP

  • Swiss-Prot No.:

    P01282-2

  • Function:

    Biomarker Proteins

  • Summary:

    VIP, or vasoactive intestinal peptide, is a neuropeptide of 28 amino acid residues that belongs to a glucagon/secretin superfamily, and it exerts its actions through three G-protein-coupled receptors (PAC1, VPAC1, and VPAC2). VIP is synthesized by trophoblast cells; it regulates trophoblast cell function and interaction with the major immune cell populations present in the pregnant uterus.

  • Form:

    Lyophilized

  • Purity:

    > 90 % as determined by SDS-PAGE.

  • Storage/Stability:

    In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
    Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

  • Storage Buffer:

    Lyophilized from sterile PBS, pH 7.4.
    Please contact us for any concerns or special requirements.
    Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
    Please refer to the specific buffer information in the hard copy of CoA.

  • Synonyms:

    PHM27 Protein, Human

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  • Amount:100 ug
  • Price:1223.00$

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