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WAVE1 Rabbit Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E28PT4897

  • Amount:

    100ul

  • Price:

    255$

  • Altername:

    WASF1; KIAA0269; SCAR1; WAVE1; Wiskott-Aldrich syndrome protein family member 1; WASP family protein member 1; Protein WAVE-1; Verprolin homology domain-containing protein 1

  • Gene Name:

    WASF1

  • Protein Name:

    Wiskott-Aldrich syndrome protein family member 1

  • Applications:

    WB,IHC,IF,ELISA

  • Reactivity:

    Human,Mouse,Rat

  • Concentration:

    1 mg/ml

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

  • Human Gene Id:

    8936

  • Swiss-Prot No.:

    Q92558

  • Mouse Gene Id:

    83767

  • Mouse Swiss Prot No:

    Q8R5H6

  • Rat Gene Id:

    294568

  • Rat Swiss Prot No:

    Q5BJU7

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human WAVE1. AA range:91-140

  • Specificity:

    WAVE1 Polyclonal Antibody detects endogenous levels of WAVE1 protein.

  • Recommended Dilutions:

    WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

  • Predicted Molecular Weight:

    70kD

  • Form of Antibody:

    Liquid

  • Storage/Stability:

    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

  • Source:

    Polyclonal, Rabbit,IgG

  • Background:

    The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

  • Tissue Expression:

    Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

  • Cellular localization:

    Cytoplasm, cytoskeleton . Cell junction, synapse . Cell junction, focal adhesion . Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas (PubMed:9889097). Partial translocation to focal adhesion sites might be mediated by interaction with SORBS2 (PubMed:18559503). In neurons, colocalizes with activated NTRK2 after BDNF addition in endocytic sites through the association with TMEM108 (By similarity). .

  • Signaling Pathway:

    Adherens_Junction;Fc gamma R-mediated phagocytosis;Regulates Actin and Cytoskeleton;

  • Function:

    domain:Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.,function:Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.,similarity:Belongs to the SCAR/WAVE family.,similarity:Contains 1 WH2 domain.,subcellular location:Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.,subunit:Component of the WAVE1 complex composed of ABI2, CYFIP2, C3orf10/HSPC300, NCKAP1 and WASF1/WAVE1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 (By similarity). Binds actin and the Arp2/3 complex. Interacts with BAIAP2.,tissue specificity:Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.,

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunofluorescence analysis of COS7 cells, using WAVE1 Antibody. The picture on the right is blocked with the synthesized peptide.

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  • Amount:100ul
  • Price:255.00$

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