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SCAP (4S6)Mouse Monoclonal Antibody(N-term)
Datasheet   

  • Catalog Number:

    E28PB3753

  • Amount:

    100ul

  • Price:

    255$

  • Altername:

    Sterol regulatory element-binding protein cleavage-activating protein, SCAP, SREBP cleavage-activating protein, SCAP, KIAA0199

  • Applications:

    WB

  • Reactivity:

    Human, Mouse

  • Swiss-Prot No.:

    Q12770

  • Specificity:

    This SCAP antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 49-83 amino acids from the N-terminal region of human SCAP.

  • Recommended Dilutions:

    WB~~1:4000

  • Predicted Molecular Weight:

    140kDa

  • Form of Antibody:

    Liquid

  • Storage/Stability:

    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

  • Isotype:

    IgG1,κ

  • Background:

    Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity).

  • Cellular localization:

    Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII- coated vesicle membrane {ECO:0000250|UniProtKB:P97260}; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols (By similarity) Requires the presence of SPRING1 for proper localization to endoplasmic reticulum (PubMed:32111832). {ECO:0000250|UniProtKB:P97260, ECO:0000269|PubMed:32111832}

  • All lanes : Anti-SCAP Antibody (N-term) at 1:4000 dilution
    Lane 1: HT-29 whole cell lysate
    Lane 2: LNCaP whole cell lysate


    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

    Predicted band size : 140 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

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  • Amount:100ul
  • Price:255.00$

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