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TUBB6 Rabbit Polyclonal Antibody (Center)
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  • Catalog Number:

    E28PB0978

  • Amount:

    100ul

  • Price:

    255$

  • Altername:

    Tubulin beta-6 chain, Tubulin beta class V, TUBB6

  • Applications:

    WB

  • Reactivity:

    Human

  • Human Gene Id:

    NP_115914.1

  • Swiss-Prot No.:

    Q9BUF5

  • Specificity:

    This TUBB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-166 amino acids from the Central region of human TUBB6.

  • Recommended Dilutions:

    WB~~1:1000

  • Predicted Molecular Weight:

    50kDa

  • Form of Antibody:

    Liquid

  • Storage/Stability:

    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

  • Isotype:

    Rabbit Ig

  • Background:

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

  • Tissue Expression:

    Ubiquitous. Maximal expression in breast and lung, where it represents around 10% of all beta-tubulins. Largely decreased expression in most cancerous tissues.

  • Cellular localization:

    Cytoplasm, cytoskeleton.

  • Purification:

    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

  • All lanes : Anti-TUBB6 Antibody (Center) at 1:2000 dilution
    Lane 1: K562 whole cell lysate
    Lane 2: MDA-MB-231 whole cell lysate
    Lane 3: Hela whole cell lysate
    Lane 4: A431 whole cell lysate
    Lane 5: A549 whole cell lysate
    Lane 6: U-251MG whole cell lysate
    Lane 7: MCF-7 whole cell lysate


    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

    Predicted band size : 50 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

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  • Amount:100ul
  • Price:255.00$

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