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UBE2I (19W6) Rabbit Monoclonal Antibody
Datasheet   

  • Catalog Number:

    E28M4544

  • Amount:

    100ul

  • Cite ID#:

    EN5542011

  • Price:

    295$

  • Applications:

    WB,IHC,ICC/IF,FC,IP

  • Reactivity:

    Human,Mouse,Rat

  • Type:

    Rabbit Monoclonal Antibody

  • Storage/Stability:

    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

  • Altername:

    C358B7.1; P18; UBC9; UBE2I; SUMO1 protein ligase;

  • Gene Name:

    UBE2I

  • Protein Name:

    UBC9, UBCE9

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

  • Human Gene Id:

    7329

  • Swiss-Prot No.:

    P63279

  • Immunogen:

    A synthetic peptide of human UBE2I/UBC9

  • Recommended Dilutions:

    WB: 1:1000 IHC: 1:50 ICC/IF: 1:50 FC: 1:20 IP: 1:20

  • Molecular Weight:

    18kDa

  • Predicted Molecular Weight:

    18kDa

  • Form of Antibody:

    Liquid

  • Isotype:

    Rabbit IgG

  • Tissue Expression:

    Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

  • Cellular localization:

    Nucleus. Cytoplasm Note=Mainly nuclear (By similarity). In spermatocytes, localizes in synaptonemal complexes (PubMed:8610150). Recruited by BCL11A into the nuclear body (By similarity). {ECO:0000250|UniProtKB:P63280, ECO:0000269|PubMed:8610150}

  • Description:

    The process of SUMO-1 conjugation is similar to that seen with ubiquitin and other forms of post-translational protein modification. Like ubiquitin, SUMO-1 is conjugated to its target protein by the coordinated action of ubiquitin conjugation enzymes E1, E2 and E3. Ubc9 (or ube2M) is a highly conserved, 158 amino acid protein that acts as a SUMO-1 conjugating enzyme. Ubc9 binds to target proteins through their SUMO-1-CS (consensus sequence) domains and interacts with SUMO via the structurally conserved amino-terminal domain.

  • Function:

    Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity (PubMed:26620705).

  • Western blot analysis of UBE2I expression in HeLa cell lysate.

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