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Primary Antibodies
Mouse anti-Human CD94, Purified mAb
Human,Not yet tested in other species.
FCM IF
$225/ 100Tests
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
BackgroundThe 4ADX22 monoclonal antibody reacts with human CD94, a 70 kDa type II transmembrane glycoprotein. CD94 belongs to the C-type lectin superfamily and is present as a heterodimer with NKG2 on the surface. CD94 is expressed by NK cells,a subset of gd T cells, and NKT cells and plays an important role in adhesion and activation of NK cell lineage.
Product Information
IsotypeMouse IgG1，kappa
Clone Number4ADX22
Experimental Methods1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7.Repeat step 5.
8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7.Repeat step 5.
8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
Notices1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
3.If the sample can not be timely analysis, please fixed;
4.For research use only, not for diagnostic or therapeutic use.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
3.If the sample can not be timely analysis, please fixed;
4.For research use only, not for diagnostic or therapeutic use.
Related productsE16HF094 Mouse Anti-Human CD94，FITC Conjugated mAb FCM IF
E16HP094 Mouse Anti-Human CD94, PE Conjugated mAb FCM IF
Profile of peripheral blood lymphocytes analyzed by flow cytometry, PE labeled goat anti-mouse IgG as secondary antibody staining.
E16HP094 Mouse Anti-Human CD94, PE Conjugated mAb FCM IF
Profile of peripheral blood lymphocytes analyzed by flow cytometry, PE labeled goat anti-mouse IgG as secondary antibody staining.
References1．Lazetic, S., C. Chang, et al. (1996). "Human natural killer cell receptors involved in MHC class I recognition are disulfide-linked heterodimers of CD94 and NKG2 subunits." J Immunol 157(11): 4741-5.
2．Phillips, J. H., C. Chang, et al. (1996). "CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA-A, HLA-B, and HLA-C allotypes." Immunity 5(2): 163-72.
3．Chang, C., A. Rodriguez, et al. (1995). "Molecular characterization of human CD94: a type II membrane glycoprotein related to the C-type lectin superfamily." Eur J Immunol 25(9): 2433-7.
2．Phillips, J. H., C. Chang, et al. (1996). "CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA-A, HLA-B, and HLA-C allotypes." Immunity 5(2): 163-72.
3．Chang, C., A. Rodriguez, et al. (1995). "Molecular characterization of human CD94: a type II membrane glycoprotein related to the C-type lectin superfamily." Eur J Immunol 25(9): 2433-7.
Price225$
Human peripheral blood lymphocytes analyzed with Purified CD94 IgGs-PE References
Conjugated Antibody
This product is also available conjugated. Format: E16HU094-100-Conjugate. Price: $485 / Size: 100μl.
E16HU094-100-FITC$485 / 100μlE16HU094-100-Biotin$485 / 100μlE16HU094-100-HRP$485 / 100μlE16HU094-100-AP$485 / 100μlE16HU094-100-AF350$485 / 100μlE16HU094-100-AF405M$485 / 100μlE16HU094-100-AF488$485 / 100μlE16HU094-100-AF514$485 / 100μlE16HU094-100-AF532$485 / 100μlE16HU094-100-AF555$485 / 100μlE16HU094-100-AF568$485 / 100μlE16HU094-100-AF405S$485 / 100μlE16HU094-100-AF405L$485 / 100μlE16HU094-100-AF546$485 / 100μlE16HU094-100-AF594$485 / 100μlE16HU094-100-AF610$485 / 100μlE16HU094-100-AF635$485 / 100μlE16HU094-100-AF647$485 / 100μlE16HU094-100-AF680$485 / 100μlE16HU094-100-AF700$485 / 100μlE16HU094-100-AF750$485 / 100μlE16HU094-100-AF790$485 / 100μlE16HU094-100-APC$485 / 100μlE16HU094-100-PE$485 / 100μlE16HU094-100-Cy3$485 / 100μlE16HU094-100-Cy5$485 / 100μlE16HU094-100-Cy5.5$485 / 100μlE16HU094-100-Cy7$485 / 100μlE16HU094-100-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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