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Primary Antibodies
Mouse anti-Human CD90, Purified mAb
Human,Not yet tested in other species.
FCM IF
$225/ 100Tests
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
BackgroundThe 4AeBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells,and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.
Product Information
IsotypeMouse IgG1
Clone Number4AeBio5E10
Experimental Methods1.Take 100μl 10e6 HEL Cells and add to the bottom of 5ml tube;
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
4.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
6.Repeat step 5.
7.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
4.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
6.Repeat step 5.
7.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
Notices1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
3.If the sample can not be timely analysis, please fixed;
4.For research use only, not for diagnostic or therapeutic use.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
3.If the sample can not be timely analysis, please fixed;
4.For research use only, not for diagnostic or therapeutic use.
Related productsE16HF090 Mouse Anti-Human CD90，FITC Conjugated mAb FCM IF
E16HP090 Mouse Anti-Human CD90, PE Conjugated mAb FCM IF
Profile of HEL Cells analyzed by flow cytometry, PE labeled goat anti-mouse IgG as secondary antibody staining.
E16HP090 Mouse Anti-Human CD90, PE Conjugated mAb FCM IF
Profile of HEL Cells analyzed by flow cytometry, PE labeled goat anti-mouse IgG as secondary antibody staining.
References1.Craig W, Kay R, Cutler RL, Lansdorp PM. Expression of Thy-1 on human hematopoietic progenitor cells. J Exp Med. 1993 May 1;177(5):1331-42.(5E10, mAb development, FC, WB, IP, PubMed)
2.Mayani H, Lansdorp PM. Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood. Blood. 1994 May 1;83(9):2410-7. (5E10, FC, PubMed)
3.Hung JT, Liao JH, Lin YC, Chang HY, Wu SF, Chang TH, Kung JT, Hsieh SL, McDevitt H, Sytwu HK. Immunopathogenic role of TH1 cells in autoimmune diabetes: evidence from a T1 and T2 doubly transgenic non-obese diabetic mouse model. J Autoimmun. 2005 Nov;25(3):181-92.(5E10, IHC, FC, PubMed)
2.Mayani H, Lansdorp PM. Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood. Blood. 1994 May 1;83(9):2410-7. (5E10, FC, PubMed)
3.Hung JT, Liao JH, Lin YC, Chang HY, Wu SF, Chang TH, Kung JT, Hsieh SL, McDevitt H, Sytwu HK. Immunopathogenic role of TH1 cells in autoimmune diabetes: evidence from a T1 and T2 doubly transgenic non-obese diabetic mouse model. J Autoimmun. 2005 Nov;25(3):181-92.(5E10, IHC, FC, PubMed)
Price225$
HEL Cells with Purified CD90 mAb 4AeBio5E10AD2 ,followed by anti-mouse IgGs-PE References Craig W, Kay R, Cutler RL, Lansdorp PM. Expression of Thy-1 on human hematopoietic cells. J Exp Med. 1993 May 1;177(5):1331-42.(5E10, mAb development, FC, WB, IP, PubMed) Mayani H, Lansdorp PM. Thy-1 expression is linked to functional properties of hematopoietic progenitor cells from human umbilical cord blood. Blood. 1994 May 1;83(9):2410-7.
Conjugated Antibody
This product is also available conjugated. Format: E16HU090-100-Conjugate. Price: $485 / Size: 100μl.
E16HU090-100-FITC$485 / 100μlE16HU090-100-Biotin$485 / 100μlE16HU090-100-HRP$485 / 100μlE16HU090-100-AP$485 / 100μlE16HU090-100-AF350$485 / 100μlE16HU090-100-AF405M$485 / 100μlE16HU090-100-AF488$485 / 100μlE16HU090-100-AF514$485 / 100μlE16HU090-100-AF532$485 / 100μlE16HU090-100-AF555$485 / 100μlE16HU090-100-AF568$485 / 100μlE16HU090-100-AF405S$485 / 100μlE16HU090-100-AF405L$485 / 100μlE16HU090-100-AF546$485 / 100μlE16HU090-100-AF594$485 / 100μlE16HU090-100-AF610$485 / 100μlE16HU090-100-AF635$485 / 100μlE16HU090-100-AF647$485 / 100μlE16HU090-100-AF680$485 / 100μlE16HU090-100-AF700$485 / 100μlE16HU090-100-AF750$485 / 100μlE16HU090-100-AF790$485 / 100μlE16HU090-100-APC$485 / 100μlE16HU090-100-PE$485 / 100μlE16HU090-100-Cy3$485 / 100μlE16HU090-100-Cy5$485 / 100μlE16HU090-100-Cy5.5$485 / 100μlE16HU090-100-Cy7$485 / 100μlE16HU090-100-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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