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Primary Antibodies
Mouse anti-Human CD147 Purified mAb
Human,Not yet tested in other species.
FCM IF
$225/ 100 Tests
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
BackgroundTBM6 monoclonal antibody reacts with basigin or neurothelin, a 50-60 kDa transmembrane glycoprotein, broadly expressed on cells of hematopoietic and non-hematopoietic origin. Neutrothelin is a blood-brain barrier-specific molecule. CD147 play a role in embryonal blood barrier development and a role in integrin-mediated adhesion in brain endothelia.
Product Information
Clone NumberTBM6
IsotypeMouse IgG1
Experimental Methods1. Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3. Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7. Repeat step 5.
8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3. Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7. Repeat step 5.
8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
Notices1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide
compounds in running water before discarding to avoid accumulation of potentially explosive
deposits in plumbing;
3. If the sample can not be timely analysis, please fixed;
4. For research use only, not for diagnostic or therapeutic use.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide
compounds in running water before discarding to avoid accumulation of potentially explosive
deposits in plumbing;
3. If the sample can not be timely analysis, please fixed;
4. For research use only, not for diagnostic or therapeutic use.
Related productsE16HF01471 Mouse Anti-Human CD147, FITC Conjugated mAb FCM IF
E16HP01471 Mouse Anti-Human CD147, PE Conjugated mAb FCM IF
Profile of peripheral blood lymphocytes analyzed by flow cytometry,FITC labeled goat anti-mouse IgG as secondary antibody staining.
E16HP01471 Mouse Anti-Human CD147, PE Conjugated mAb FCM IF
Profile of peripheral blood lymphocytes analyzed by flow cytometry,FITC labeled goat anti-mouse IgG as secondary antibody staining.
References1.Schlossman, SL et al., eds. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York. eds.
Price225$
Human peripheral blood lymphocytes analyzed with IgGs-FITC References
Conjugated Antibody
This product is also available conjugated. Format: E16HU01471-100-Conjugate. Price: $485 / Size: 100μl.
E16HU01471-100-FITC$485 / 100μlE16HU01471-100-Biotin$485 / 100μlE16HU01471-100-HRP$485 / 100μlE16HU01471-100-AP$485 / 100μlE16HU01471-100-AF350$485 / 100μlE16HU01471-100-AF405M$485 / 100μlE16HU01471-100-AF488$485 / 100μlE16HU01471-100-AF514$485 / 100μlE16HU01471-100-AF532$485 / 100μlE16HU01471-100-AF555$485 / 100μlE16HU01471-100-AF568$485 / 100μlE16HU01471-100-AF405S$485 / 100μlE16HU01471-100-AF405L$485 / 100μlE16HU01471-100-AF546$485 / 100μlE16HU01471-100-AF594$485 / 100μlE16HU01471-100-AF610$485 / 100μlE16HU01471-100-AF635$485 / 100μlE16HU01471-100-AF647$485 / 100μlE16HU01471-100-AF680$485 / 100μlE16HU01471-100-AF700$485 / 100μlE16HU01471-100-AF750$485 / 100μlE16HU01471-100-AF790$485 / 100μlE16HU01471-100-APC$485 / 100μlE16HU01471-100-PE$485 / 100μlE16HU01471-100-Cy3$485 / 100μlE16HU01471-100-Cy5$485 / 100μlE16HU01471-100-Cy5.5$485 / 100μlE16HU01471-100-Cy7$485 / 100μlE16HU01471-100-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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