World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
Mouse anti-Human CD14, Purified mAb
Human,Not yet tested in other species.
FCM IF
$225/ 100 Tests
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
BackgroundW4 clone reacts with CD14, a 53-55 kDa molecule. CD14 is a human high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS-endotoxin) and serum LPS-binding protein (LPB). CD14 antigen has a strong presence on the surface of monocytes/macrophages, is weakly expressed on granulocytes, but not expressed by myeloid progenitor cells. CD14 functions as a receptor for endotoxin; when the monocytes become activated they release cytokines such as TNF, and up-regulate cell surface molecules including adhesion molecules.This clone is cross reactive with non-human primate.
Product Information
IsotypeMouse IgG1
Clone NumberW4
Experimental Methods1. Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3. Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7. Repeat step 5.
8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3. Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7. Repeat step 5.
8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
Notices1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide
compounds in running water before discarding to avoid accumulation of potentially explosive
deposits in plumbing;
3. If the sample can not be timely analysis, please fixed;
4. For research use only, not for diagnostic or therapeutic use.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide
compounds in running water before discarding to avoid accumulation of potentially explosive
deposits in plumbing;
3. If the sample can not be timely analysis, please fixed;
4. For research use only, not for diagnostic or therapeutic use.
Related productsE16HF014 Mouse Anti-Human CD14，FITC Conjugated mAb FCM IF
E16HP014 Mouse Anti-Human CD14, PE Conjugated mAb FCM IF
E16HC014 Mouse Anti-Human CD14，PE-Cy5 Conjugated mAb FCM IF
E16HA014 Mouse Anti-Human CD14, APC Conjugated mAb FCM IF
E16HB014 Mouse Anti-Human CD14，Biotin Conjugated mAb FCM IF
Profile of peripheral blood granulocytes analyzed by flow cytometry, PE labeled goat anti-mouse IgG as secondary antibody staining.
E16HP014 Mouse Anti-Human CD14, PE Conjugated mAb FCM IF
E16HC014 Mouse Anti-Human CD14，PE-Cy5 Conjugated mAb FCM IF
E16HA014 Mouse Anti-Human CD14, APC Conjugated mAb FCM IF
E16HB014 Mouse Anti-Human CD14，Biotin Conjugated mAb FCM IF
Profile of peripheral blood granulocytes analyzed by flow cytometry, PE labeled goat anti-mouse IgG as secondary antibody staining.
References1. Knapp, W et al., eds. (1989) Leucocyte Typing IV : White Cell Differentiation Antigens, Oxford University Press, New York.
2. Makgoba, MW et al., (1988) Nature 331: 86-88
3. Boyd. AW et al. (1989) Blood 73: 1896-1903
2. Makgoba, MW et al., (1988) Nature 331: 86-88
3. Boyd. AW et al. (1989) Blood 73: 1896-1903
Price225$
Human peripheral blood monocytes analyzed with Purified References
Conjugated Antibody
This product is also available conjugated. Format: E16HU014-100-Conjugate. Price: $485 / Size: 100μl.
E16HU014-100-FITC$485 / 100μlE16HU014-100-Biotin$485 / 100μlE16HU014-100-HRP$485 / 100μlE16HU014-100-AP$485 / 100μlE16HU014-100-AF350$485 / 100μlE16HU014-100-AF405M$485 / 100μlE16HU014-100-AF488$485 / 100μlE16HU014-100-AF514$485 / 100μlE16HU014-100-AF532$485 / 100μlE16HU014-100-AF555$485 / 100μlE16HU014-100-AF568$485 / 100μlE16HU014-100-AF405S$485 / 100μlE16HU014-100-AF405L$485 / 100μlE16HU014-100-AF546$485 / 100μlE16HU014-100-AF594$485 / 100μlE16HU014-100-AF610$485 / 100μlE16HU014-100-AF635$485 / 100μlE16HU014-100-AF647$485 / 100μlE16HU014-100-AF680$485 / 100μlE16HU014-100-AF700$485 / 100μlE16HU014-100-AF750$485 / 100μlE16HU014-100-AF790$485 / 100μlE16HU014-100-APC$485 / 100μlE16HU014-100-PE$485 / 100μlE16HU014-100-Cy3$485 / 100μlE16HU014-100-Cy5$485 / 100μlE16HU014-100-Cy5.5$485 / 100μlE16HU014-100-Cy7$485 / 100μlE16HU014-100-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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