World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
Mouse anti-Human CD7, Purified mAb
Human,Not yet tested in other species.
FCM IF
$225/ 100Tests
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
BackgroundThe 4AeBio124-1D1 monoclonal antibody reacts with human CD7, also known as gp40 and Leu9. CD7, a 40 kD receptor, is a member of the immunoglobulin gene superfamily. The N-terminal amino acid sequence (aa1-107) is highly homologous to Ig kappa light chain sequence; while the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T lymphocytes, and T cell leukemias. It is also found on natural killer cells, a small suppopulation of normal B cells and on maligant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity, as measured by calcium flux, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.A clonogenic subpopulation of human CD34(+) CD38(-) cord blood cells that express CD45RA and HLA-DR and high levels of the CD7 has been reported. These cells possess the capacity for lymphopoiesis. They can generate B-cells, natural killer cells, and dendritic cells but do not possess the capacity to develop into myeloid cells or erythroid cells. The CD7(+) phenotype distinguishes primitive human lymphoid progenitors from pluripotent stem cells.Furthermore, it has been suggested that CD7 co-operates with CD28 during Treg function, as mice deficient in both CD28 and CD7 have reduced total numbers of Tregs and these Tregs have reduced suppressive activity.
Product Information
IsotypeMouse IgG1
Clone Number4AUCHT2
Experimental Methods1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7.Repeat step 5.
8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7.Repeat step 5.
8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
Notices1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
3.If the sample can not be timely analysis, please fixed;
4.For research use only, not for diagnostic or therapeutic use.
Profile of peripheral blood lymphocytes analyzed by flow cytometry, PE labeled goat anti-mouse IgG as secondary antibody staining.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
3.If the sample can not be timely analysis, please fixed;
4.For research use only, not for diagnostic or therapeutic use.
Profile of peripheral blood lymphocytes analyzed by flow cytometry, PE labeled goat anti-mouse IgG as secondary antibody staining.
Price225$
blood lymphocytes stained with Purified CD7 mAb 4AeBio124-1D1 IgGs-PE
Conjugated Antibody
This product is also available conjugated. Format: E16HU007-100-Conjugate. Price: $485 / Size: 100μl.
E16HU007-100-FITC$485 / 100μlE16HU007-100-Biotin$485 / 100μlE16HU007-100-HRP$485 / 100μlE16HU007-100-AP$485 / 100μlE16HU007-100-AF350$485 / 100μlE16HU007-100-AF405M$485 / 100μlE16HU007-100-AF488$485 / 100μlE16HU007-100-AF514$485 / 100μlE16HU007-100-AF532$485 / 100μlE16HU007-100-AF555$485 / 100μlE16HU007-100-AF568$485 / 100μlE16HU007-100-AF405S$485 / 100μlE16HU007-100-AF405L$485 / 100μlE16HU007-100-AF546$485 / 100μlE16HU007-100-AF594$485 / 100μlE16HU007-100-AF610$485 / 100μlE16HU007-100-AF635$485 / 100μlE16HU007-100-AF647$485 / 100μlE16HU007-100-AF680$485 / 100μlE16HU007-100-AF700$485 / 100μlE16HU007-100-AF750$485 / 100μlE16HU007-100-AF790$485 / 100μlE16HU007-100-APC$485 / 100μlE16HU007-100-PE$485 / 100μlE16HU007-100-Cy3$485 / 100μlE16HU007-100-Cy5$485 / 100μlE16HU007-100-Cy5.5$485 / 100μlE16HU007-100-Cy7$485 / 100μlE16HU007-100-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
Categories by research area
Categories by product type
- Antibodies
- Primary Antibodies
- Tag antibodies
- Internal control antibodies
- Pathological (Immunohistochemistry, IHC) antibodies
- Cell Signal Transduction Research Antibodies
- Secondary Antibody
- Antibody Sampler Kits
- ELISA Kits
- Recombinant Proteins
- Fluorescence Reagents
- Signal Inhibitors and Activators
- Transfection Reagent
- Cell biology kits
- Overexpression Cell Lines
- Cell Lysates
- Purification Resins
- 2019-nCoV & Monkeypox