World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
Mouse anti-Human CD49f, PE Conjugated mAb
Human,Not yet tested in other species.
FCM IF
$255/ 100Tests
QuantityTotal: $255
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
BackgroundThe 4AeBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA‐6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA‐6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The 4AeBioGoH3 antibody is cross‐reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.
Product Information
IsotypeRat IgG2a
Clone Number4AeBioGoH3
Experimental Methods1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
Notices1.This reagent has been pre-diluted for use at the recommended Volume per Test.
We typically use 1 X 106 cells in a 100μl experimental sample (a test);
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
4. If the sample can not be timely analysis, please fixed;
5. For research use only, not for diagnostic or therapeutic use.
We typically use 1 X 106 cells in a 100μl experimental sample (a test);
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
4. If the sample can not be timely analysis, please fixed;
5. For research use only, not for diagnostic or therapeutic use.
Related productsE16HU0491 Mouse Anti-Human CD49f，Purified mAb FCM IF
E16HF0491 Mouse Anti-Human CD49f，FITC Conjugated mAb FCM IF
E16HF0491 Mouse Anti-Human CD49f，FITC Conjugated mAb FCM IF
References1.Sonnenberg A, Daams H, Van der Valk MA, Hilkens J, Hilgers J. Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin. J Histochem Cytochem. 1986 Aug;34(8):1037‐46. (GoH3, FC, IH/F PubMed)
2.Aumailley M, Timpl R, Sonnenberg A. Antibody to integrin alpha 6 subunit specifically inhibits cell‐binding to laminin fragment 8. Exp Cell Res.1990 May;188(1):55‐60. (GoH3, FA, PubMed)
3.Ambrose HE, Wagner SD. Alpha6‐integrin is expressed on germinal centre B cells and modifies growth of a B‐cell line. Immunology. 2004 Apr;111(4):400‐6. (GoH3, FC, PubMed)
2.Aumailley M, Timpl R, Sonnenberg A. Antibody to integrin alpha 6 subunit specifically inhibits cell‐binding to laminin fragment 8. Exp Cell Res.1990 May;188(1):55‐60. (GoH3, FA, PubMed)
3.Ambrose HE, Wagner SD. Alpha6‐integrin is expressed on germinal centre B cells and modifies growth of a B‐cell line. Immunology. 2004 Apr;111(4):400‐6. (GoH3, FC, PubMed)
Price255$
Conjugated Antibody
This product is also available conjugated. Format: E16HP0491-100-Conjugate. Price: $485 / Size: 100μl.
E16HP0491-100-FITC$485 / 100μlE16HP0491-100-Biotin$485 / 100μlE16HP0491-100-HRP$485 / 100μlE16HP0491-100-AP$485 / 100μlE16HP0491-100-AF350$485 / 100μlE16HP0491-100-AF405M$485 / 100μlE16HP0491-100-AF488$485 / 100μlE16HP0491-100-AF514$485 / 100μlE16HP0491-100-AF532$485 / 100μlE16HP0491-100-AF555$485 / 100μlE16HP0491-100-AF568$485 / 100μlE16HP0491-100-AF405S$485 / 100μlE16HP0491-100-AF405L$485 / 100μlE16HP0491-100-AF546$485 / 100μlE16HP0491-100-AF594$485 / 100μlE16HP0491-100-AF610$485 / 100μlE16HP0491-100-AF635$485 / 100μlE16HP0491-100-AF647$485 / 100μlE16HP0491-100-AF680$485 / 100μlE16HP0491-100-AF700$485 / 100μlE16HP0491-100-AF750$485 / 100μlE16HP0491-100-AF790$485 / 100μlE16HP0491-100-APC$485 / 100μlE16HP0491-100-PE$485 / 100μlE16HP0491-100-Cy3$485 / 100μlE16HP0491-100-Cy5$485 / 100μlE16HP0491-100-Cy5.5$485 / 100μlE16HP0491-100-Cy7$485 / 100μlE16HP0491-100-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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