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Primary Antibodies
Mouse anti-Human CD73, FITC Conjugated mAb
Human,Not yet tested in other species.
FCM IF
$225/ 100Tests
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
BackgroundThis 4AAD2 monoclonal antibody reacts with human CD73, a 5’-ectonucleotidase that converts 5’-adenosine monophosphate to adenosine. CD73 is expressed on the surface of endothelial cells, as well as B and T cells, including some CD4+Foxp3+ regulatory T cells. Adenosine production by these cells has been linked to the inhibition of CD4 T cell effector functions such as proliferation and cytokine secretion.
Product Information
IsotypeMouse IgG1
Clone Number4AAD2
Experimental Methods1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
Notices1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
3.If the sample can not be timely analysis, please fixed;
4.For research use only, not for diagnostic or therapeutic use.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
3.If the sample can not be timely analysis, please fixed;
4.For research use only, not for diagnostic or therapeutic use.
Related productsE16HU073 Mouse Anti-Human CD73, Purified mAb FCM IF
E16HP073 Mouse Anti-Human CD73, PE Conjugated mAb FCM IF
E16HP073 Mouse Anti-Human CD73, PE Conjugated mAb FCM IF
References1. Deaglio S, Dwyer KM, Gao W, Friedman D, Usheva A, Erat A, Chen JF, Enjyoji K, Linden J, Oukka M, Kuchroo VK, Strom TB, Robson SC. Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression. J Exp Med. 2007 Jun 11;204(6):1257-65.
2.Kobie JJ, Shah PR, Yang L, Rebhahn JA, Fowell DJ, Mosmann TR. T regulatory and primed uncommitted CD4 T cells express CD73, which suppresses effector CD4 T cells by converting 5'-adenosine monophosphate to adenosine. J Immunol. 2006 Nov 15;177(10):6780-6.
3.Nakamura T, Kubagawa H, Ohno T, Cooper MD. Characterization of an IgM Fc-binding receptor on human T cells. J Immunol. 1993 Dec 15;151(12):6933-41. (4AAD2, FC, Pubmed)
2.Kobie JJ, Shah PR, Yang L, Rebhahn JA, Fowell DJ, Mosmann TR. T regulatory and primed uncommitted CD4 T cells express CD73, which suppresses effector CD4 T cells by converting 5'-adenosine monophosphate to adenosine. J Immunol. 2006 Nov 15;177(10):6780-6.
3.Nakamura T, Kubagawa H, Ohno T, Cooper MD. Characterization of an IgM Fc-binding receptor on human T cells. J Immunol. 1993 Dec 15;151(12):6933-41. (4AAD2, FC, Pubmed)
Price225$
Conjugated Antibody
This product is also available conjugated. Format: E16HF073-100-Conjugate. Price: $485 / Size: 100μl.
E16HF073-100-FITC$485 / 100μlE16HF073-100-Biotin$485 / 100μlE16HF073-100-HRP$485 / 100μlE16HF073-100-AP$485 / 100μlE16HF073-100-AF350$485 / 100μlE16HF073-100-AF405M$485 / 100μlE16HF073-100-AF488$485 / 100μlE16HF073-100-AF514$485 / 100μlE16HF073-100-AF532$485 / 100μlE16HF073-100-AF555$485 / 100μlE16HF073-100-AF568$485 / 100μlE16HF073-100-AF405S$485 / 100μlE16HF073-100-AF405L$485 / 100μlE16HF073-100-AF546$485 / 100μlE16HF073-100-AF594$485 / 100μlE16HF073-100-AF610$485 / 100μlE16HF073-100-AF635$485 / 100μlE16HF073-100-AF647$485 / 100μlE16HF073-100-AF680$485 / 100μlE16HF073-100-AF700$485 / 100μlE16HF073-100-AF750$485 / 100μlE16HF073-100-AF790$485 / 100μlE16HF073-100-APC$485 / 100μlE16HF073-100-PE$485 / 100μlE16HF073-100-Cy3$485 / 100μlE16HF073-100-Cy5$485 / 100μlE16HF073-100-Cy5.5$485 / 100μlE16HF073-100-Cy7$485 / 100μlE16HF073-100-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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