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Primary Antibodies
Mouse anti-Human CD69, FITC Conjugated mAb
Human,Not yet tested in other species.
FCM IF
$225/ 100Tests
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
BackgroundThe 4AFN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.
Product Information
IsotypeMouse IgG1
Clone Number4AFN50
Experimental Methods1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
Notices1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
3.If the sample can not be timely analysis, please fixed;
4.For research use only, not for diagnostic or therapeutic use.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
3.If the sample can not be timely analysis, please fixed;
4.For research use only, not for diagnostic or therapeutic use.
Related productsE16HU069 Mouse Anti-Human CD69，Purified mAb FCM IF
E16HP069 Mouse Anti-Human CD69, PE Conjugated mAb FCM IF
Profile of peripheral blood lymphocytes analyzed by flow cytometry
E16HP069 Mouse Anti-Human CD69, PE Conjugated mAb FCM IF
Profile of peripheral blood lymphocytes analyzed by flow cytometry
References1.Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
2.Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
2.Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Price225$
FITC Staining of overnight PHA-stimulated normal human peripheral blood cells
Conjugated Antibody
This product is also available conjugated. Format: E16HF069-100-Conjugate. Price: $485 / Size: 100μl.
E16HF069-100-FITC$485 / 100μlE16HF069-100-Biotin$485 / 100μlE16HF069-100-HRP$485 / 100μlE16HF069-100-AP$485 / 100μlE16HF069-100-AF350$485 / 100μlE16HF069-100-AF405M$485 / 100μlE16HF069-100-AF488$485 / 100μlE16HF069-100-AF514$485 / 100μlE16HF069-100-AF532$485 / 100μlE16HF069-100-AF555$485 / 100μlE16HF069-100-AF568$485 / 100μlE16HF069-100-AF405S$485 / 100μlE16HF069-100-AF405L$485 / 100μlE16HF069-100-AF546$485 / 100μlE16HF069-100-AF594$485 / 100μlE16HF069-100-AF610$485 / 100μlE16HF069-100-AF635$485 / 100μlE16HF069-100-AF647$485 / 100μlE16HF069-100-AF680$485 / 100μlE16HF069-100-AF700$485 / 100μlE16HF069-100-AF750$485 / 100μlE16HF069-100-AF790$485 / 100μlE16HF069-100-APC$485 / 100μlE16HF069-100-PE$485 / 100μlE16HF069-100-Cy3$485 / 100μlE16HF069-100-Cy5$485 / 100μlE16HF069-100-Cy5.5$485 / 100μlE16HF069-100-Cy7$485 / 100μlE16HF069-100-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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