World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
Mouse anti-Human CD34, PE-Cy5 Conjugated mAb.
Human,Not yet tested in other species.
FC IF
$255/ 100Tests
QuantityTotal: $255
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
Background4A43A1 reacts with CD34 molecule, a 105-120 kDa heavily O-glycosylated transmembrane glycoprotein expressed on hematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. The intracellular chain of the CD34 antigen is a target for phosphorylation by activated protein kinase C suggesting that CD34 may play a role in signal transduction. CD34 may play a role in adhesion of specific antigens to endothelium. Clone 4A43A1 belongs to the class II epitope. * CD34 mAb is useful for detection and saparation of hematopoietic stem cells.
Product Information
IsotypeMouse IgG3
Clone Number4A43A1
Experimental Methods1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
Notices1.This reagent has been pre-diluted for use at the recommended Volume per Test.
We typically use 1 X 106 cells in a 100μl experimental sample (a test);
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
4. If the sample can not be timely analysis, please fixed;
5. For research use only, not for diagnostic or therapeutic use.
We typically use 1 X 106 cells in a 100μl experimental sample (a test);
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
4. If the sample can not be timely analysis, please fixed;
5. For research use only, not for diagnostic or therapeutic use.
Related productsE16HU0341 Mouse Anti-Human CD34, Purified mAb FC IF
E16HF0341 Mouse Anti-Human CD34, FITC Conjugated mAb FC IF
E16HP0341 Mouse Anti-Human CD34, PE Conjugated mAb FC IF
E16HB0341 Mouse Anti-Human CD34,Biotin Conjugated mAb FC IF
Profile of peripheral blood lymphocytes analyzed by flow cytometry
E16HF0341 Mouse Anti-Human CD34, FITC Conjugated mAb FC IF
E16HP0341 Mouse Anti-Human CD34, PE Conjugated mAb FC IF
E16HB0341 Mouse Anti-Human CD34,Biotin Conjugated mAb FC IF
Profile of peripheral blood lymphocytes analyzed by flow cytometry
References1. Schlossman, SL et al., eds. (1995) Leucocyte Typing V : White Cell Differentiation Antigens, Oxford University Press, New York.
2. Freeman SD et al., (1995) Blood 85 : 2005
3. Thomas R and Lipsky PE . (1994) J Immunol 153: 4016
4.Nakamura Y. et al., ( 1994 ) Blood 83:1442
2. Freeman SD et al., (1995) Blood 85 : 2005
3. Thomas R and Lipsky PE . (1994) J Immunol 153: 4016
4.Nakamura Y. et al., ( 1994 ) Blood 83:1442
Price255$
Human peripheral blood lymphocytes analyzed with CD45-FITC，CD34-PE-CY5 mAb
Conjugated Antibody
This product is also available conjugated. Format: E16HC0341-100-Conjugate. Price: $485 / Size: 100μl.
E16HC0341-100-FITC$485 / 100μlE16HC0341-100-Biotin$485 / 100μlE16HC0341-100-HRP$485 / 100μlE16HC0341-100-AP$485 / 100μlE16HC0341-100-AF350$485 / 100μlE16HC0341-100-AF405M$485 / 100μlE16HC0341-100-AF488$485 / 100μlE16HC0341-100-AF514$485 / 100μlE16HC0341-100-AF532$485 / 100μlE16HC0341-100-AF555$485 / 100μlE16HC0341-100-AF568$485 / 100μlE16HC0341-100-AF405S$485 / 100μlE16HC0341-100-AF405L$485 / 100μlE16HC0341-100-AF546$485 / 100μlE16HC0341-100-AF594$485 / 100μlE16HC0341-100-AF610$485 / 100μlE16HC0341-100-AF635$485 / 100μlE16HC0341-100-AF647$485 / 100μlE16HC0341-100-AF680$485 / 100μlE16HC0341-100-AF700$485 / 100μlE16HC0341-100-AF750$485 / 100μlE16HC0341-100-AF790$485 / 100μlE16HC0341-100-APC$485 / 100μlE16HC0341-100-PE$485 / 100μlE16HC0341-100-Cy3$485 / 100μlE16HC0341-100-Cy5$485 / 100μlE16HC0341-100-Cy5.5$485 / 100μlE16HC0341-100-Cy7$485 / 100μlE16HC0341-100-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
Categories by research area
Categories by product type
- Antibodies
- Primary Antibodies
- Tag antibodies
- Internal control antibodies
- Pathological (Immunohistochemistry, IHC) antibodies
- Cell Signal Transduction Research Antibodies
- Secondary Antibody
- Antibody Sampler Kits
- ELISA Kits
- Recombinant Proteins
- Fluorescence Reagents
- Signal Inhibitors and Activators
- Transfection Reagent
- Cell biology kits
- Overexpression Cell Lines
- Cell Lysates
- Purification Resins
- 2019-nCoV & Monkeypox