World supplier of antibodies, antigens and kits since 2007
Primary Antibodies
Mouse anti-Human CD14, APC Conjugated mAb.
Human, Not yet tested in other species.
FC FA IHC IF
$255/ 100Tests
QuantityTotal: $255
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Form Or Antibody
Form of AntibodyPhosphate-buffered solution, pH 7.4, containing 0.09% sodium azide and 0.2% (w/v) BSA
Storage/StabilityStore at 4°C.DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.
BackgroundThe 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.
Product Information
IsotypeMouse IgG1
Clone Number4A61D3
Experimental Methods1. 1.Take 100μl peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
2.Add 10μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 ℃ then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3; Cat.: FXP005]
[Cell fixation: 2% formaldehyde solution]
Notices1.1.This reagent has been pre-diluted for use at the recommended Volume per Test.
We typically use 1 X 106 cells in a 100μl experimental sample (a test);
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
4.If the sample can not be timely analysis, please fixed;
5.For research use only, not for diagnostic or therapeutic use.
We typically use 1 X 106 cells in a 100μl experimental sample (a test);
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing;
4.If the sample can not be timely analysis, please fixed;
5.For research use only, not for diagnostic or therapeutic use.
Related productsE16HU014 Mouse Anti-Human CD14, Purified mAb FC FA IHC IF
E16HP014 Mouse Anti-Human CD14, PE Conjugated mAb FC FA IHC IF
E16HC014 Mouse Anti-Human CD14, PE-Cy5 Conjugated mAb FC FA IHC IF
E16HF014 Mouse Anti-Human CD14, FITC Conjugated mAb FC FA IHC IF
E16HB014 Mouse Anti-Human CD14, Biotin Conjugated mAb FC FA IHC IF
E16HP014 Mouse Anti-Human CD14, PE Conjugated mAb FC FA IHC IF
E16HC014 Mouse Anti-Human CD14, PE-Cy5 Conjugated mAb FC FA IHC IF
E16HF014 Mouse Anti-Human CD14, FITC Conjugated mAb FC FA IHC IF
E16HB014 Mouse Anti-Human CD14, Biotin Conjugated mAb FC FA IHC IF
References1. Knapp, W et al., eds. (1989) Leucocyte Typing IV : White Cell Differentiation Antigens, Oxford University Press, New York.
2. Makgoba, MW et al., (1988) Nature 331: 86-88
3. Boyd. AW et al. (1989) Blood 73: 1896-1903
2. Makgoba, MW et al., (1988) Nature 331: 86-88
3. Boyd. AW et al. (1989) Blood 73: 1896-1903
Price255$
PHA and ConA activated lymphocytes analyzed with PE CD14 mAb
Conjugated Antibody
This product is also available conjugated. Format: E16HA014-100-Conjugate. Price: $485 / Size: 100μl.
E16HA014-100-FITC$485 / 100μlE16HA014-100-Biotin$485 / 100μlE16HA014-100-HRP$485 / 100μlE16HA014-100-AP$485 / 100μlE16HA014-100-AF350$485 / 100μlE16HA014-100-AF405M$485 / 100μlE16HA014-100-AF488$485 / 100μlE16HA014-100-AF514$485 / 100μlE16HA014-100-AF532$485 / 100μlE16HA014-100-AF555$485 / 100μlE16HA014-100-AF568$485 / 100μlE16HA014-100-AF405S$485 / 100μlE16HA014-100-AF405L$485 / 100μlE16HA014-100-AF546$485 / 100μlE16HA014-100-AF594$485 / 100μlE16HA014-100-AF610$485 / 100μlE16HA014-100-AF635$485 / 100μlE16HA014-100-AF647$485 / 100μlE16HA014-100-AF680$485 / 100μlE16HA014-100-AF700$485 / 100μlE16HA014-100-AF750$485 / 100μlE16HA014-100-AF790$485 / 100μlE16HA014-100-APC$485 / 100μlE16HA014-100-PE$485 / 100μlE16HA014-100-Cy3$485 / 100μlE16HA014-100-Cy5$485 / 100μlE16HA014-100-Cy5.5$485 / 100μlE16HA014-100-Cy7$485 / 100μlE16HA014-100-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
Categories by research area
Categories by product type
- Antibodies
- Primary Antibodies
- Tag antibodies
- Internal control antibodies
- Pathological (Immunohistochemistry, IHC) antibodies
- Cell Signal Transduction Research Antibodies
- Secondary Antibody
- Antibody Sampler Kits
- ELISA Kits
- Recombinant Proteins
- Fluorescence Reagents
- Signal Inhibitors and Activators
- Transfection Reagent
- Cell biology kits
- Overexpression Cell Lines
- Cell Lysates
- Purification Resins
- 2019-nCoV & Monkeypox