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CD28 (Phospho-Tyr218) Antibody
Datasheet   

  • Catalog Number:

    E11-0845A

  • Amount:

    100μg

  • Swiss-Prot No.:

    P10747

  • Other Names:

    T-cell-specific surface glycoprotein CD28 precursor, TP44

  • All Sites:

    Human: Tyr218; Mouse: Tyr216

  • Storage/Stability:

    Store at -20℃/1 year

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human CD28 around the phosphorylation site of tyrosine 218 (A-A-YP-R-S).

  • Purification:

    The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    CD28 (Phospho-Tyr218) antibody detects endogenous levels of CD28 only when phosphorylated at tyrosine 218.

  • Reactivity:

    Human, Mouse

  • Applications:

    WB: 1:500~1:1000 ELISA: 1:40000

  • References:

    Aruffo A., Proc. Natl. Acad. Sci. U.S.A. 84:8573-8577(1987).
    Lee K.P., J. Immunol. 145:344-352(1990).
    Deshpande M., Hum. Immunol. 63:20-23(2002).
    --------

  • Western blot analysis of extracts from HepG2 cells, treated with nocodazole (1ug/ml, 16hours), using CD28 (Phospho-Tyr218) antibody.

  • Price:

    225$

  • Research Area:

    Autophagy antibody Cardiovascular Cancer Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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