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ERK1/2 (Phospho-Thr202/Tyr204) AntibodyPDF

  • Catalog Number:

    E012017-1

  • Amount:

    50μg/50μl

  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

  • Storage/Stability:

    Store at -20℃/1 year

  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human ERK1/2 around the phosphorylation site of Thr202/Tyr204 (F-L-TP-E -YP-V-A).

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

  • Specificity/Sensitivity:

    ERK1/2 (phospho-Thr202/Tyr204) antibody detects endogenous levels of ERK1/2 only when phosphorylated at Thr202/Tyr204.

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000

  • Swiss-Prot No.:

    P27361

  • References:

    Marais, R. et al. (1993) Cell 73, 381-93.
    Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
    Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
    Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
    Pagès G (2007) J Cell Physiol 213, 454-63
    Basbous J, et al. (2007) Mol Cell Biol 27, 3936-50

  • P-Peptide - +
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ERK1/2 (phospho- Thr202/Tyr204) (E012017).

  • Western blot analysis of extracts from Jurkat cells treated with PMA. Left: Using ERK1/2 (phospho- Thr202/Tyr204) antibody (E012017). Right: Using the same antibody preincubated with synthesized peptide.

  • Price:

    145$

  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:50μg/50μl
  • Price:145.00$

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