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Akt (Phospho-Ser473) AntibodyPDF

  • Catalog Number:

    E011054-1
  • Amount:

    50μg/50μl
  • Form of Antibody:

    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage/Stability:

    Store at -20℃/1 year
  • Immunogen:

    The antiserum was produced against synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S).
  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
  • Specificity/Sensitivity:

    Akt (phospho-Ser473) antibody detects endogenous levels of Akt only when phosphorylated at serine 473.
  • Reactivity:

    Human, Mouse, Rat
  • Applications:

    WB: 1:500~1:1000 IHC: 1:50~1:100
  • Swiss-Prot No.:

    P31749
  • References:

    Baudhuin LM, et al. (2004) FASEB J Feb; 18(2): 341-3.
    Min YH, et al. (2004) Cancer Res; 64(15): 5225-31.
    Feng J, et al. (2004) J Biol Chem; 279(34): 35510-7.
  • P-Peptide - +
    Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using Akt (phospho-Ser473) antibody (E011054).
  • Western blot analysis of extract from HeLa cells untreated or treated with heat shock using Akt (Ab-473) antibody (E021054, Lane 1 and 2) and Akt (phospho-Ser473) antibody (E011054, Lane 3 and 4).
  • Price:

    145$
  • Research Area:

    Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells

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Catalog Number

  • Amount:50μg/50μl
  • Price:145.00$

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